it makes no difference whatsoever, even 4x has still lots of bandwidth left to push even something like quad 7970s, I suggest you check out Linus Tech Tips video benchmarking these lanes at x4, x8 and x16, theoretically, a single card at x16 compared to two, 50% equivalents of the single, will only impact the performance by maybe a frame or two, which in a real life scenario is nothing really